With the rise of cell and gene therapy, new molecules and delivery vectors such as plasmid DNA, mRNA, and viral vectors (AAV/LV) have emerged. For the purification of large-sized molecules and viral vectors, traditional filler microspheres face disadvantages such as slow processing speed, low effective binding capacity, low yield and resolution; while the improvement of downstream purification processes can reduce drug production costs and improve drug accessibility. has important significance. Purification carriers in the form of convection columns have multiple advantages in the purification of macromolecules in the CGT field and can provide better solutions, so they are increasingly used.

Convection mechanism: Efficient mass transfer coefficient, its combined loading capacity and separation effect are not affected by molecular size and retention time, fast flow rate, and short process time.
Low shear force - small back pressure: The flow channel length of the convection column is generally about 2cm, the permeability coefficient is high, and the back pressure during the treatment process is low, which can achieve a higher operating flow rate; at the same time, the fluid presents laminar flow in the convection column, without turbulence , no local vortex, lower shear force.
High resolution: Since the peak width and resolution of traditional packings are limited by the effective molecular diffusion coefficient and loading speed, and the high viscosity and small diffusion coefficient of ultra-large molecules result in poor resolution; convection column purification is not limited by diffusion and can be used in precision It has obvious advantages in pure impurity removal.

Shape flow direction: tubular/radial flow

Specification: 1.25ml

Ligand type: polythymidine

Binding capacity: 1-2mg /ml (~4000nt mRNA)

Pore diameter: 2-3μm

Interface type: 10-32UNF, 1/16" tube connection

Recommended flow rate :2-3CV/min

Maximum pressure: 2MPa

Working temperature: 4‒ 40℃

Recommended PH: Working: 2‒12

Chemical stability: can tolerate 6M guanidine hydrochloride, 6M urea, 1M acetic acid, 0.1M sodium hydroxide, 50% ethanol

Storage conditions: 2℃‒25℃; 20% ethanol

Shelf life: 1 year